MD Anderson Cancer Center Research Assistant II in Houston, Texas
The mission of The University of Texas M. D. Anderson Cancer Center is to eliminate cancer in Texas, the nation, and the world through outstanding programs that integrate patient care, research and prevention, and through education for undergraduate and graduate students, trainees, professionals, employees and the public.
The primary purpose of the Research Assistant II is to apply technical expertise and experience to plan, coordinate, design, and carry out research projects in the Department of Cancer Systems Imaging.
JOB SPECIFIC COMPETENCIES
Animals: Maintain and expand mouse colonies. Plan and execute multiple crosses to generate new genetic animal models. Administer various treatments and diets to animals. Candidate would know how to perform oral gavages, i.p. injection, subcutaneous injections for subcutaneous tumor growth, tail vein catheterizations, and necropsy procedures. Monitor animals and be able to assess physiological and pathological changes in mouse models. Be able to administer and prepare samples for metabolic assays. Perform xenograft experiments. Analyze resultant data and prepare results for dissemination to laboratory. Candidate would successfully complete training and certification to handle and work with live animals, primarily mice, in a safe and humane manner following institutional guidelines. Candidate would be responsible for mouse colony maintenance by setting up breeders, weaning as needed, tail snipping, genotyping using PCR, monitoring health and welfare of animals, and efficiently maintaining animal inventory records. Assisting fellow laboratory personnel with animal experiments such as imaging and surgeries. Stores and analyzes animal tissue and keep records of ongoing data for future experiments.
Tissue Culture: Must have excellent tissue culture technique and be able to maintain and store cell lines under sterile and healthy conditions. Candidate should follow ATCC guideline for cell culture and cryogenic storage. Candidate must have the ability to observe and identify cell changes under the microscope, determine cell counts and cell viability. Utilize aseptic techniques and biosafety measures in experimental work.
Basic Techniques: Molecular biology and cancer biology. Candidate will extract metabolites from tissue and cell culture and generate experimental samples for analysis by mass spectrometry. Isolates, purifies and analyzes RNA, DNA and protein including hybridization, gel electrophoresis, staining, performing PCR reactions, quantitative RT-PCR assays, DNA sequencing and assays for signal transduction. Take pictures of cells or tissue on the light or fluorescence microscope. Prepares slides, graphs and tables via photography, computer or manually. Communicate results to the principal investigator and share in group presentations of these data as well as promote ongoing projects in the laboratory. Assist with compilation of data for presentation or publication. Perform other laboratory duties as requested.
Apply basic background in science to the application of laboratory techniques, using judgment, analysis and evaluation. Read literature and confer with superiors regarding new procedures. Master essential skills and techniques used in the laboratory. Develop new and novel procedures for carrying out assigned research by reading literature and conferring with peers and superiors; implement these new procedures. Recordkeeping and data collection required on all experiments performed.
Maintain all documentation. Compile, write and submit project results to superiors. Assist in developing new and novel procedures for carrying out assigned research by reading literature and conferring with peers and superiors; implement these new procedures. Recordkeeping and data collection required on all experimental protocols performed. Perform other duties as assigned
Other duties as assigned
Education: Bachelor's degree with major course works in one of the basic sciences.
Preferred Education: Master's Level Degree
Experience: Two years of experience in scientific or experimental research work. No experience required with preferred degree.
Preferred Experience: No experience with preferred degree.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html