MD Anderson Cancer Center Research Assistant II - Translational Molecular Pathology in Houston, Texas

Summary

The primary purpose of the Research Assistant II position is to provide technical and coordination skills in carrying out various research projects as they pertain to the implementation of new technologies, and performing routine in vivo and in vitro experiments related to cancer therapeutic development. Working with mice is a must. This position is also responsible for learning, operating and maintaining laboratory equipment, keeping accurate records and evaluating data in accordance with methods requested or established in SOPs.

Key Functions

·Performance of all required laboratory tasks and experiments that include cell biological experiments like maintenance of cell lines, performing cell proliferation, invasion, cell death & apoptosis assays; Biochemical and molecular experiments like DNA/RNA, metabolite & protein isolation, cloning, western blot analysis, qRT-PCR, immunoprecipitations, & flow cytometry;

·Pipetting and preparation of large number of samples for high-throughput screens.

·Learning specialized experiments like Seahorse Extracellular Flux analysis, processing patient derived and xenograft tissues for Seahorse analysis, and for preparation of IHC samples. Other novel experiments as needed.

· Learning and working with routine analytical software, and generating graphs and figures of data.

·If necessary, learning to perform in vivo experiments in mice which include tumor generation, surgical implantation of tumors, drug administration, tumor measurements, tissue harvesting and processing

·Operation of laboratory apparatus and equipment. Preparation of stock solutions and buffers. Maintenance of laboratory supplies and purchasing necessary reagents for experiments in a timely manner.

·Maintain a clear record of all experimental work performed. Ensures that records are properly maintained and consistently reflect the current status of the lab work. Keeps and updates records of cell lines, analytical and tissue samples collected. Keeps and updates records of cell lines generated in the laboratory, received from other laboratories or cell lines being frozen or thawed in our lab.

·Performance of experiments as directed by supervisor, within the time frame specified. Transportation of samples and Performance of experiments in off-site locations when needed. Other duties, as assigned.

Bachelor's degree with major course works in one of the basic sciences. Two years experience in scientific or experimental research work. No experience required with preferred degree. It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html