MD Anderson Cancer Center Research Assistant II - Mouse Handling in Houston, Texas
The department of Leukemia is dedicated to quality patient care, innovative research, and developing more effective treatments for all types of leukemia. Clinical research conducted by our faculty and staff consistently translates into new leukemia therapies and advances overall knowledge of the disease.
The Research Assistant II position provides technical and coordination skills in carrying out various research projects.
The ideal candidate will have animal handling and flow cytometry experience.
The salary range is: min-$34,400, mid-$43,000 and max-$51,600.
Animal work experience or willing to learn the technique in maintaining animal colonies, including overseeing breeding, genotyping, and record keeping for all animal strains; Assist with animal work under supervision– dosing(oral gavage, IP or IV), tissue preparation and sample processing
· Maintains and establishes tissue culture cell lines which require good sterile techniques and the ability to observe and identify cell changes under the microscope, determine cell counts on the hemocytometer or coulter counter, performs colony counts or determine cell viability.
· Monitors, and shelves tissue culture supplies to ensure efficient functioning. Prepare cells for cryogenic storage and reestablishment by the use of small vials, dry ice and liquid nitrogen.
· Assist with cell biology (transient and stable engineering of cell lines, also using viral transduction),
· Assist with immunological techniques using flow cytometry and ELISA
- Molecular Techniques:
· Isolates, purifies and analyzes, RNA, DNA and protein including hybridization, gel electrophoresis autoradiography, ECL staining, performing PCR reactions, quantitative RT-PCR assay, DNA sequencing and assays for signal transducing enzymes.
· Purification and conjugation of immunoglobulins. Should have basic knowledge of solution and buffer preparation in which ionic concentration and pH is critical. Should have basic training in use and safety of radioisotopes. Assist with molecular biology (molecular cloning, transient and stable gene silencing)
- Laboratory Equipment and Maintenance:
· Performs routine maintenance of ultra-low freezers, liquid nitrogen, C02 and water levels. Should be able to maintain equipment such as high-speed centrifuges, pH meters and microfuges.
· Manually operates and maintains the lab equipment. Visually reads equipment operation manuals and procedure manual whenever necessary. Must be able to lift and carry items up to 20-25 pounds and carry or move from one area of the lab to another by using a roller cart. Must be able to comply with all institutional safety policies including Universal Precautions and OSHA standards when handling toxic, hazardous, flammable, radioactive or potentially infectious or carcinogenic materials. This may require wearing common protective garments or equipment such as gloves, eye protection, face mask, gown or lab coat, etc. Additionally some procedures may require the use of a fume hood or biological safety cabinet.
Required: Bachelor's degree with major course works in one of the basic sciences.
Required: Two years experience in scientific or experimental research work. No experience required with preferred degree.
Preferred: Previous animal handling and flow cytometry experience.
It is the policy of The University of Texas MD Anderson Cancer Center to provide equal employment opportunity without regard to race, color, religion, age, national origin, sex, gender, sexual orientation, gender identity/expression, disability, protected veteran status, genetic information, or any other basis protected by institutional policy or by federal, state or local laws unless such distinction is required by law. http://www.mdanderson.org/about-us/legal-and-policy/legal-statements/eeo-affirmative-action.html